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to look at the physiological result of hypoxia on differentiation of ASC, a collagen gel contraction assay was utilized, which measures the adjust in gel measurement with time.
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To validate the ability of differentiated ASCs to contract by induction with carbachol, one-mobile contraction assay was performed at twenty% O2.
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Tukey’s check of importance was Employed in the submit-hoc Evaluation. knowledge are introduced as mean ± normal mistake (SE). All experiments have been carried out with two ASC samples and 1 SMC sample and repeated not less than 3 times.
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